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Registros recuperados: 55 | |
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Mazurais, David; Coves, Denis; Papandroulakis, Nikos; Ortega, Aurelio; Desbruyeres, Elisabeth; Huelvan, Christine; Le Gall, Marie-madeleine; De La Gandara, Fernando; Cahu, Chantal. |
The aim of this study was to determine whether mortality observed during the larval development of reared bluefin tuna (Thunnus thynnus) could be related to improper expression profiles of key genes involved in digestive or antioxidant response capabilities. Tuna larvae were sampled at hatching, 2, 5, 10, 15 and 20 dph (days post hatching) for the relative quantification of transcripts encoded by genes involved in digestive [trypsinogen 1 (TRYP1), alpha-amylase (AMY), aminopeptidase N (ANPEP)] and antioxidant [catalase (CAT)] functions. The levels of expression of ANPEP related to the development and maturation of intestinal function increased from 5 to 20 dph. Furthermore, AMY and TRYP1 genes, which are pancreatic enzymes implicated in carbohydrate and... |
Tipo: Text |
Palavras-chave: Tuna; Gene; Expression; Development; Digestion; Antioxidant. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00171/28264/26521.pdf |
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Natalini,Cláudio Corrêa; Linardi,Renata Lehn. |
P-glycoprotein (P-gp) is a membrane transporter encoded in the Multi-drug Resistance (MDR1) gene expressed in several normal tissues and over expressed in tumor cells. P-gp was already identified in different species but not yet in equine. MDR1 gene and P-gp are able to interfere with bioavailability and disposition of several drugs, altering pharmacokinetic and pharmacodinamic of drugs. The presence of the MDR1 and P-gp in the central nervous system blocks the entry of certain drugs in this tissue and reduces drug absorption and enhances drug elimination when P-gp and MDR1 are presented in the gastrointestinal tract. This study showed that the MDR1 gene is present in equine ileum. Future studies on the impact of the P-glycoprotein encoded gene MDR1 on... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular biology; Opioids; Equine; Gene; MDR1; ABCB1. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000100048 |
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Morga, Benjamin. |
The history of French oyster culture shows very well how fragile this farming is to stock overexploitation and diseases. The production of flat oyster, Ostrea edulis, fell dramatically after the appearance of two parasitic diseases: arteiliosis and bonamiosis. The curative means against bonamiosis (Bonamia ostreae) are restricted and imply a good knowledge of the parasite's life cycle and of its interaction with its host. Studying the expression of the parasite's and the host's genes during the infection seems essential in providing new adapted measures in order to maintain, if not relaunch the flat oyster culture. In this context, this thesis aims to understand the flat oyster-parasite interactions, and especially, the molecular basis of the resistance to... |
Tipo: Text |
Palavras-chave: Biologie moléculaire; Gene; Parasite; Huîtres; Ostrea edulis. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3369.pdf |
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Ropert,C.. |
Gene therapy is an active field that has progressed rapidly into clinical trials in a relatively short time. The key to success for any gene therapy strategy is to design a vector able to serve as a safe and efficient gene delivery vehicle. This has encouraged the development of nonviral DNA-mediated gene transfer techniques such as liposomes. Many liposome-based DNA delivery systems have been described, including molecular components for targeting given cell surface receptors or for escaping from the lysosomal compartment. Another recent technology using cationic lipids has been evaluated and has generated substantial interest in this approach to gene transfer. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene; PH-sensitive liposome; Cationic lipid; Transfection. |
Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200004 |
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Fleury, Elodie; Huvet, Arnaud. |
Summer mortality of Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. A high heritability was estimated for resistance to summer mortality, which provided an opportunity to develop lines of oysters that were resistant (R) or susceptible (S) to summer mortality. Previous genome-wide expression profiling study of R and S oyster gonads highlighted reproduction and antioxidant defense as constitutive pathways that operate differentially between these two lines. Here, we show that signaling in innate immunity also operates differentially between these lines, and we hypothesize that this is at the main determinant of their difference in survival in the field. A reanalysis of our published microarray data... |
Tipo: Text |
Palavras-chave: Mollusca; Crassostrea gigas; Gene; Immunity; CDNA microarray; Summer mortality. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19423/17213.pdf |
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Bacca, Helene; Huvet, Arnaud; Fabioux, Caroline; Daniel, Jean-yves; Delaporte, Maryse; Pouvreau, Stephane; Van Wormhoudt, A; Moal, Jeanne. |
To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes... |
Tipo: Text |
Palavras-chave: Reproduction; Regulation; Oyster; Glycogen; Expression; Gene; Energy; Crassostrea gigas; Bivalve. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-435.pdf |
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PANTIC,IGOR; PANTIC,SENKA; PAUNOVIC,JOVANA; PEROVIC,MILAN. |
Grey level co-occurrence matrix analysis (GLCM) is a well-known mathematical method for quantification of cell and tissue textural properties, such as homogeneity, complexity and level of disorder. Recently, it was demonstrated that this method is capable of evaluating fine structural changes in nuclear structure that otherwise are undetectable during standard microscopy analysis. In this article, we present the results indicating that entropy, angular second moment, variance, and texture correlation of lymphocyte nuclear structure determined by GLCM method are different in thymus cortex when compared to medulla. A total of 300 thymus lymphocyte nuclei from 10 one-month-old mice were analyzed: 150 nuclei from cortex and 150 nuclei from medullar regions of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Texture analysis; GLCM matrix; CD4; CD8; Gene. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301063 |
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MAGALHAES, J. V.; CARVALHO JÚNIOR, G. A. de; SCHAFFERT, R. E.; LANDAU, E. C.; MIRANDA, R. A. de; VIANA, J. H. M.; MENEZES, C. B. de; SILVA, L. A.; GUIMARAES, C. T.; COELHO, A. M.. |
bitstream/item/159487/1/circ-218.pdf |
Tipo: Circular Técnica (INFOTECA-E) |
Palavras-chave: Sorgo; Solo; Toxidez; Alumínio; Gene; Melhoramento genético vegetal. |
Ano: 2016 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1063395 |
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Biffi,CP; Stefani,LM; Miletti,LC; Matiello,CA; Backes,RG; Almeida,JM; Neves,GB. |
Isolates of Salmonella sp (104) from poultry samples were isolated and serotyped where eleven were identified as Salmonella Typhimurium (ST). ST isolates were phenotypically tested by disk diffusion and minimum inhibitory concentration (MIC). Four genes related to enrofloxacin (GyrA, GyrB, ParC and ParE), two to gentamicin (AadA and AadB) and two to ceftiofur (BlaCMY-2 and AmpC) resistance were searched by PCR. Our results showed ST resistance to all three antibiotics tested (18.1% for ceftiofur, 45.4% for gentamicin, and 18.1% for enrofloxacin) according to the diffusion test. In the MIC test, the ST isolates showed higher levels of resistance (27.2% for ceftiofur, 54.5% for gentamicin, and 18.2% for enrofloxacin). Three resistance genes out of four... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial; Gene; Resistance; Salmonella. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000200013 |
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Fagundes,RBS; Picoli,EAT; Lana,UGP; Valicente,FH. |
Bacillus thuringiensis harbors genes encoding Cry proteins found in chromosomes or plasmids of different sizes (4-150 Mb). Although the smaller plasmids are more abundant in B. thuringiensis, their specific function is unknown. As for the megaplasmids, their main recognized function is to harbor cry genes, although the sequencing of some of these plasmids indicates the occurrence of other important genes. This work used a new protocol for practical and rapid extraction of plasmid DNA in order to characterize the plasmid patterns of Brazilian strains belonging to Embrapa Milho e Sorgo research center B. thuringiensis bank. We tried to further assess the relationship of plasmid patterns with strains belonging to the same serovars and strains causing 100% and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterium; Cry protein; Gene; Megaplasmid. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2011000500012 |
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Registros recuperados: 55 | |
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